Mutant Rep protein of the porcine circovirus type 2 N-glycosylation:23-25aa, 256-258aa mutation reduced virus replication but 286-288aa mutation enhanced virus replication in PK-15 cells

Jianli Shi; Zhe Peng; Fang Fu; Shaojian Xu; Shengnan Xu; Xiaoyan Cong; Xiaoyuan Yuan; Jiang Yu; Jiaqiang Wu; Wenbo Sun; Yijun Du; Jun Li; Jinbao Wang

doi:10.1016/j.vetmic.2015.03.016

Mutant Rep protein of the porcine circovirus type 2 N-glycosylation:23-25aa, 256-258aa mutation reduced virus replication but 286-288aa mutation enhanced virus replication in PK-15 cells

Vet Microbiol. 2015 Jun 12;177(3-4):370-2. doi: 10.1016/j.vetmic.2015.03.016. Epub 2015 Mar 23.

Authors

Jianli Shi¹, Zhe Peng¹, Fang Fu², Shaojian Xu¹, Shengnan Xu³, Xiaoyan Cong¹, Xiaoyuan Yuan¹, Jiang Yu¹, Jiaqiang Wu¹, Wenbo Sun¹, Yijun Du¹, Jun Li⁴, Jinbao Wang⁵

Affiliations

¹ Division of Swine Diseases, Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, No.8 Sangyuan Road, Jinan 250100, China.
² Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
³ College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong 266109, China.
⁴ Division of Swine Diseases, Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, No.8 Sangyuan Road, Jinan 250100, China. Electronic address: junli79@163.com.
⁵ Division of Swine Diseases, Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, No.8 Sangyuan Road, Jinan 250100, China. Electronic address: wangjb@saas.ac.cn.

PMID: 25829242
DOI: 10.1016/j.vetmic.2015.03.016

Abstract

Porcine circovirus type 2 (PCV2) Rep protein and the splice variant Rep' protein impact genome replication. The Rep protein contains three potential N-glycosylation at positions 23-25aa (NPS), 256-258aa (NQT) and 286-288aa (NAT). Three double copy infectious clones with Rep protein N-glycosylation at positions mutations 23-25aa (DPS), 256-258aa (DQT) and 286-288aa (DAT) were constructed and their function in virus replication in PK-15 cells was investigated. The results showed that the double copy infectious clone with N-glycosylation site mutation could be rescued in vitro and 23-25aa, 256-258aa mutation reduced virus replication but 286-288aa mutation enhanced virus replication.

Keywords: Infectious clone; Mutationat; N-Glycosylation; Porcine circovirus type 2; Rescued.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Circovirus / genetics
Circovirus / physiology*
DNA Replication
Fluorescent Antibody Technique
Genome, Viral / genetics
Genome, Viral / physiology
Glycosylation
Mutant Proteins / genetics
Mutant Proteins / physiology*
Mutation
Protein Isoforms / genetics
Protein Isoforms / physiology
Swine
Viral Proteins / genetics
Viral Proteins / physiology*
Virus Replication* / genetics

Substances

Mutant Proteins
Protein Isoforms
Viral Proteins