Human OCT2 variant c.808G>T confers protection effect against cisplatin-induced ototoxicity

Pharmacogenomics. 2015;16(4):323-32. doi: 10.2217/pgs.14.182.

Abstract

Aim: Assuming that genetic variants of the SLC22A2 and SLC31A1 transporter affect patients' susceptibility to cisplatin-induced ototoxicity, we compared the distribution of 11 SLC22A2 variants and the SLC31A1 variant rs10981694 between patients with and without cisplatin-induced ototoxicity.

Patients & methods: Genotyping was performed in 64 pediatric patients and significant findings were re-evaluated in 66 adults.

Results: The SLC22A2 polymorphism rs316019 (c.808G>T; Ser270Ala) was significantly associated with protection from cisplatin-induced ototoxicity in the pediatric (p = 0.022) and the adult cohort (p = 0.048; both: Fisher's exact test). This result was confirmed by multiple logistic regression analysis accounting for age which was identified as a relevant factor for ototoxicity as well (rs316019: OR [G/T vs G/G] = 0.12, p = 0.009; age: OR [per year]: 0.84, p = 0.02).

Conclusion: These results identified rs316019 as potential pharmacogenomic marker for cisplatin-induced ototoxicity and point to a critical role of SLC22A2 for cisplatin transport in humans and its contribution to the organ specific side effects of this drug. Original submitted 17 September 2014; Revision submitted 19 December 2014.

Keywords: SLC22A2; SLC31A1; cisplatin; ototoxicity; polymorphism.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adolescent
  • Adult
  • Cation Transport Proteins / genetics*
  • Child
  • Child, Preschool
  • Cisplatin / administration & dosage
  • Cisplatin / adverse effects*
  • Copper Transporter 1
  • Drug-Related Side Effects and Adverse Reactions / genetics
  • Female
  • Genetic Association Studies
  • Genotype
  • Haplotypes
  • Humans
  • Male
  • Neoplasms / drug therapy*
  • Neoplasms / genetics
  • Neoplasms / pathology
  • Organic Cation Transport Proteins / genetics*
  • Organic Cation Transporter 2
  • Polymorphism, Single Nucleotide

Substances

  • Cation Transport Proteins
  • Copper Transporter 1
  • Organic Cation Transport Proteins
  • Organic Cation Transporter 2
  • SLC22A2 protein, human
  • SLC31A1 protein, human
  • Cisplatin