Liquid-liquid extraction and liquid chromatography-mass spectrometry detection of curcuminoids from bacterial culture medium

Suryani Tan; Thusitha W T Rupasinghe; Dedreia L Tull; Mary Ann Augustin; Sally L Gras

doi:10.1016/j.jchromb.2015.02.024

Liquid-liquid extraction and liquid chromatography-mass spectrometry detection of curcuminoids from bacterial culture medium

J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Apr 15:988:116-20. doi: 10.1016/j.jchromb.2015.02.024. Epub 2015 Feb 24.

Authors

Suryani Tan¹, Thusitha W T Rupasinghe², Dedreia L Tull², Mary Ann Augustin³, Sally L Gras⁴

Affiliations

¹ CSIRO Food, Nutrition and Bioproducts Flagship, 671 Sneydes Road, Werribee, Vic 3030, Australia; Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Vic 3010, Australia; Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Vic 3010, Australia.
² Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Vic 3010, Australia; Metabolomics Australia, The University of Melbourne, Parkville, Vic 3010, Australia.
³ CSIRO Food, Nutrition and Bioproducts Flagship, 671 Sneydes Road, Werribee, Vic 3030, Australia.
⁴ Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Vic 3010, Australia; Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Vic 3010, Australia; ARC Dairy Innovation Hub, The University of Melbourne, Parkville, Vic 3010, Australia. Electronic address: sgras@unimelb.edu.au.

PMID: 25770788
DOI: 10.1016/j.jchromb.2015.02.024

Abstract

Liquid chromatography-mass spectrometry (LC-MS) has been used to detect polyphenolic curcuminoids found in turmeric but studies of metabolism by bacterial and mammalian cells in vitro are compromised by poor recovery from the culture medium. We report a liquid-liquid extraction procedure with ethyl acetate and use LC-MS to quantify extracted curcuminoids. Ethyl acetate allows recoveries of ∼ 80-86% of curcuminoids from the bacterial growth medium, bacterial cell lysate and combined bacterial cell and growth medium matrices; a clear improvement over acetonitrile where recoveries were ∼ 25-66%. This optimised method will enable studies of curcuminoid metabolism and may be applicable to other hydrophobic polyphenolic compounds.

Keywords: Cell medium; Curcumin; Curcuminoids; Ethyl acetate; LC–MS; Recovery.

MeSH terms

Bioreactors / microbiology
Chromatography, Liquid / methods*
Curcumin / analysis
Curcumin / isolation & purification*
Curcumin / metabolism
Escherichia / metabolism*
Linear Models
Liquid-Liquid Extraction / methods*
Mass Spectrometry / methods*
Polyphenols / analysis
Polyphenols / isolation & purification*
Polyphenols / metabolism

Substances

Polyphenols
Curcumin