Direct sequencing of a disulfide-linked peptide with electrospray ionization tandem mass spectrometry

Analyst. 2015 Apr 21;140(8):2623-7. doi: 10.1039/c5an00011d.

Abstract

Dissociation of disulfide is normally mandatory prior to disulfide peptide sequencing via electrospray ionization collision induced dissociation mass spectrometry (ESI-CID-MS). Herein, a facile method for directly sequencing intact disulfide peptides was proposed. The basic principles involved were electrolyte-enhanced corona discharge in ESI and the following oxidative cleavage reaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Disulfides / chemistry*
  • Humans
  • Insulin / chemistry
  • Molecular Sequence Data
  • Oxidation-Reduction
  • Peptides / chemistry*
  • Sequence Analysis, Protein / methods*
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Tandem Mass Spectrometry / methods*

Substances

  • Disulfides
  • Insulin
  • Peptides