Abstract
We studied the feasibility of using halloysite clay nanotubes (HNTs) and carboxyl-functionalised multi-walled carbon nanotubes (COOH-MWCNTs) as antigen carriers to improve immune responses against a recombinant LipL32 protein (rLipL32). Immunisation using the HNTs or COOH-MWCNTs significantly increased the rLipL32-specific IgG antibody titres (p < 0.05) of Golden Syrian hamsters. None of the vaccines tested conferred protection against a challenge using a virulent Leptospira interrogans strain. These results demonstrated that nanotubes can be used as antigen carriers for delivery in hosts and the induction of a humoral immune response against purified leptospiral antigens used in subunit vaccine preparations.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aluminum Silicates / immunology*
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Animals
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Antigens / administration & dosage
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Antigens, Bacterial / administration & dosage
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Antigens, Bacterial / immunology
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Bacterial Outer Membrane Proteins / immunology*
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Bacterial Vaccines / immunology*
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Carbon Dioxide / immunology
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Clay
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Cricetinae
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Feasibility Studies
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Immunity, Humoral / immunology
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Immunoglobulin G / analysis*
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Leptospira interrogans / classification
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Leptospira interrogans / immunology*
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Lipoproteins / immunology*
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Mesocricetus
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Nanotubes, Carbon*
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Recombinant Proteins / administration & dosage
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Recombinant Proteins / immunology
Substances
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Aluminum Silicates
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Antigens
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Antigens, Bacterial
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Bacterial Outer Membrane Proteins
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Bacterial Vaccines
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Immunoglobulin G
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LipL32 protein, Leptospira
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Lipoproteins
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Nanotubes, Carbon
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Recombinant Proteins
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Carbon Dioxide
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carboxyl radical
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Clay