Transcriptomics using axolotls

Methods Mol Biol. 2015:1290:309-19. doi: 10.1007/978-1-4939-2495-0_24.

Abstract

Microarray and RNA-sequencing technology now exists for the characterization of the Ambystoma mexicanum transcriptome. With sufficient replication, these tools give the opportunity to truly investigate gene expression in a variety of experimental paradigms. Analysis of data from the Amby002 array and RNA-sequencing technology can identify genes that change expression levels in concert with each other, which in turn may reveal mechanisms associated with biological processes and molecular functions.

MeSH terms

  • Ambystoma mexicanum / genetics*
  • Animals
  • Cluster Analysis
  • Gene Expression Profiling / methods*
  • Molecular Sequence Annotation
  • Oligonucleotide Array Sequence Analysis
  • RNA / genetics
  • RNA / isolation & purification
  • Sequence Analysis, RNA

Substances

  • RNA