Activation of peripheral blood T cells via the p75 interleukin 2 receptor

J Exp Med. 1989 Oct 1;170(4):1445-50. doi: 10.1084/jem.170.4.1445.

Abstract

By using mAb and flow cytometry, a constitutive expression of the p75 IL-2R was revealed in human peripheral blood CD8+ T cells and TCR delta-1+ T cells as well as in CD16+ NK cells. Anti-p75 IL-2R mAb almost completely inhibited the induction of cytolytic activity in these T cells by brief exposure to IL-2, as estimated by anti-TCR/CD3 mAb-targeted cytotoxicity. While anti-p55 IL-2R mAb alone inhibited the response only modestly, maximal inhibition was achieved by combining both anti-p55 and anti-p75 IL-2R mAbs. These results indicate that the p75 IL-2R constitutively expressed on peripheral blood CD8+ T cells and TCR delta-1+ T cells is predominantly responsible for the direct activation of these cells by IL-2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Antigen-Antibody Reactions
  • CD4-Positive T-Lymphocytes / physiology
  • Cytotoxicity, Immunologic
  • Humans
  • In Vitro Techniques
  • Interleukin-2 / pharmacology
  • Lymphocyte Activation*
  • Molecular Weight
  • Receptors, Interleukin-2 / physiology*
  • Receptors, Interleukin-2 / ultrastructure
  • T-Lymphocytes / classification
  • T-Lymphocytes / physiology*

Substances

  • Antibodies, Monoclonal
  • Interleukin-2
  • Receptors, Interleukin-2