(1)H, (13)C and (15)N backbone and side-chain resonance assignment of the LAM-RRM1 N-terminal module of La protein from Dictyostelium discoideum

Christos T Chasapis; Aikaterini I Argyriou; Maria Apostolidi; Parthena Konstantinidou; Detlef Bentrop; Constantinos Stathopoulos; Georgios A Spyroulias

doi:10.1007/s12104-015-9597-z

(1)H, (13)C and (15)N backbone and side-chain resonance assignment of the LAM-RRM1 N-terminal module of La protein from Dictyostelium discoideum

Biomol NMR Assign. 2015 Oct;9(2):303-7. doi: 10.1007/s12104-015-9597-z. Epub 2015 Feb 18.

Authors

Christos T Chasapis¹, Aikaterini I Argyriou¹, Maria Apostolidi², Parthena Konstantinidou², Detlef Bentrop³, Constantinos Stathopoulos², Georgios A Spyroulias⁴

Affiliations

¹ Department of Pharmacy, University of Patras, 26504, Patras, Greece.
² Department of Biochemistry, School of Medicine, University of Patras, 26504, Patras, Greece.
³ Institute of Physiology II, University of Freiburg, 79104, Freiburg, Germany.
⁴ Department of Pharmacy, University of Patras, 26504, Patras, Greece. G.A.Spyroulias@upatras.gr.

PMID: 25687647
DOI: 10.1007/s12104-015-9597-z

Abstract

The N-terminal half of La protein consists of two concatenated motifs: La motif (LAM) and the N-terminal RNA recognition motif (RRM1) both of which are responsible for poly(U) RNA binding. Here, we present the backbone and side-chain assignments of the (1)H, (13)C and (15)N resonances of the 191-residue LAM-RRM1 region of the La protein from the lower eukaryote Dictyostelium discoideum and its secondary structure prediction.

Keywords: La protein; NMR spectroscopy; RNA binding; RNA polymerase III; Recombinant protein expression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbon-13 Magnetic Resonance Spectroscopy*
Dictyostelium / metabolism*
Nitrogen Isotopes
Protein Structure, Tertiary
Proton Magnetic Resonance Spectroscopy*
Protozoan Proteins / chemistry*

Substances

Nitrogen Isotopes
Protozoan Proteins