The DNA polymorphisms at the seven restriction sites in the beta-globin gene cluster in healthy Koreans were examined using four restriction endonucleases, Hinc II, Hind III, Ava II, and Bam HI. Seven (f = 0.326) and four individuals (f = 0.246) were homozygous for [+-----+] and [+----+-], respectively, among 66 individuals examined. As to the subhaplotypes 5' to the delta-globin gene, 25 (f = 0.615) and three individuals (f = 0.213) were homozygous for [+----] and [-+-++], respectively. The frequency of [-++-+], which carries the A gamma T-globin gene, may be low in this population. It was recognized again that [+/+] at the Hinc II site 5' to the epsilon-globin gene always accompanied [--+--] at the Hinc II sites in and 3' to the psi beta 1-globin gene. The Korean, Japanese, and Chinese populations were not significantly different from each other in their haplotypes (subhaplotypes). The frequencies of the abnormal alpha- and gamma-globin gene arrangements in Koreans were low. alpha-Thalassemia may occur at low frequency in Koreans.