A light-inducible CRISPR-Cas9 system for control of endogenous gene activation

Nat Chem Biol. 2015 Mar;11(3):198-200. doi: 10.1038/nchembio.1753. Epub 2015 Feb 9.

Abstract

Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR-Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • CRISPR-Cas Systems / drug effects*
  • CRISPR-Cas Systems / radiation effects
  • Cryptochromes / genetics
  • DNA / genetics
  • Gene Expression Regulation / genetics
  • Gene Expression Regulation / radiation effects
  • Humans
  • Light
  • Optogenetics
  • Plasmids / genetics
  • Protein Engineering
  • RNA / biosynthesis
  • RNA / genetics
  • Streptococcus pyogenes / enzymology
  • Streptococcus pyogenes / genetics
  • Transcription, Genetic / drug effects
  • Transcriptional Activation / drug effects*
  • Transcriptional Activation / radiation effects*

Substances

  • CRY2 protein, human
  • Cryptochromes
  • RNA
  • DNA