An artificial CO-releasing metalloprotein built by histidine-selective metallation

Chem Commun (Camb). 2015 Mar 7;51(19):3993-6. doi: 10.1039/c4cc10204e.

Abstract

We report the design and synthesis of an aquacarbonyl Ru(II) dication cis-[Ru(CO)2(H2O)4](2+) reagent for histidine (His)-selective metallation of interleukin (IL)-8 at site 33. The artificial, non-toxic interleukin (IL)-8-Ru(II)(CO)2 metalloprotein retained IL-8-dependent neutrophil chemotactic activity and was shown to spontaneously release CO in live cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomimetic Materials / chemistry*
  • Chemotaxis
  • HeLa Cells
  • Histidine / chemistry*
  • Humans
  • Interleukin-8 / chemistry
  • Metalloproteins / chemistry*
  • Neutrophils / cytology
  • Ruthenium / chemistry*

Substances

  • Interleukin-8
  • Metalloproteins
  • Histidine
  • Ruthenium