Clinical utility comparison of two benchtop deep sequencing instruments for rapid diagnosis of newly emergent influenza infections

X Ren; Y Hu; F Yang; Y Xiao; L Li; J Dong; L Sun; Y Zhu; Q Jin

doi:10.1016/j.cmi.2014.10.016

Clinical utility comparison of two benchtop deep sequencing instruments for rapid diagnosis of newly emergent influenza infections

Clin Microbiol Infect. 2015 Mar;21(3):290.e1-4. doi: 10.1016/j.cmi.2014.10.016. Epub 2014 Oct 29.

Authors

X Ren¹, Y Hu¹, F Yang¹, Y Xiao¹, L Li¹, J Dong¹, L Sun¹, Y Zhu¹, Q Jin²

Affiliations

¹ MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
² MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. Electronic address: zdsys@vip.sina.com.

PMID: 25658538
DOI: 10.1016/j.cmi.2014.10.016

Abstract

Rapid determination of full genomes of influenza A viruses from clinical samples is vital to outbreak preparedness. We benchmarked Ion Torrent PGM and Illumina MiSeq to the standard Sanger sequencing on two real clinical samples and demonstrated their performance for full-genome determination and polymorphism identification.

Keywords: Deep sequencing; Ion Torrent PGM; MiSeq; genome sequencing; influenza.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Genome, Viral
Genotype
High-Throughput Nucleotide Sequencing / instrumentation*
High-Throughput Nucleotide Sequencing / methods*
Humans
Influenza A virus / classification
Influenza A virus / genetics*
Influenza, Human / diagnosis*
Influenza, Human / virology*
Mutation
Polymorphism, Single Nucleotide
Reproducibility of Results