A liver-specific nuclear protein that binds to the distal promoter element of the rat tyrosine aminotransferase gene

FEBS Lett. 1989 Jan 30;243(2):318-22. doi: 10.1016/0014-5793(89)80153-x.

Abstract

Using a gel retardation assay and exonuclease III footprinting, we have analyzed sequence-specific DNA-binding nuclear factors which interact with the distal promoter element of the rat tyrosine aminotransferase gene. A factor called LspA1, binding to a sequence that resembles the consensus binding site for the transcription factor Ap-1, was shown to be present in adult rat-liver nuclear protein extracts but not in the extracts from embryonic liver or spleen nuclei.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites
  • DNA-Binding Proteins / metabolism*
  • Embryo, Mammalian / metabolism
  • Exodeoxyribonucleases
  • Gels
  • Liver / analysis*
  • Liver / embryology
  • Molecular Sequence Data
  • Nuclear Proteins / metabolism*
  • Promoter Regions, Genetic*
  • Rats
  • Restriction Mapping
  • Tyrosine Transaminase / genetics*

Substances

  • DNA-Binding Proteins
  • Gels
  • Nuclear Proteins
  • Tyrosine Transaminase
  • Exodeoxyribonucleases
  • exodeoxyribonuclease III