Renalase protects against contrast-induced nephropathy in Sprague-Dawley rats

PLoS One. 2015 Jan 30;10(1):e0116583. doi: 10.1371/journal.pone.0116583. eCollection 2015.

Abstract

Background: Contrast-induced nephropathy (CIN) is the third leading cause of hospital-acquired acute renal failure. Oxidative stress, apoptosis and inflammation play crucial roles in CIN. Renalase is a newly discovered monoamine oxidase from the kidney. We hypothesize that renalase could protect against CIN through anti-oxidation, anti-inflammation and anti-apoptosis pathways.

Methods: We tested our hypothesis in vivo with a rat model of Ioversol-induced CIN and in vitro. Sprague-Dawley rats were divided into 4 groups (n = 6 per group): control group, Ioversol group (rats subjected to Ioversol-induced CIN), Ioversol plus vehicle group (CIN rats pretreated with vehicle) and Ioversol plus renalase group (CIN rats pretreated with 2 mg/kg recombinant renalase). HK2 cells were treated with Ioversol or H2O2.

Results: The results showed that pretreatment with renalase attenuated the deterioration of renal function, tubular necrosis, oxidative stress, apoptosis and inflammation (P<0.05). Furthermore, renalase protected HK2 cells against the cytotoxicity of Ioversol and suppressed Caspase-3 activity, oxidative stress and apoptosis induced by H2O2.

Conclusion: Recombinant renalase protected CIN in rats through anti-oxidation, anti-apoptosis and anti-inflammation mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Blood Urea Nitrogen
  • Contrast Media / adverse effects*
  • Creatinine / blood
  • Inflammation / pathology
  • Kidney / drug effects
  • Kidney / pathology
  • Kidney Diseases / blood
  • Kidney Diseases / chemically induced*
  • Kidney Diseases / drug therapy*
  • Kidney Diseases / pathology
  • Male
  • Monoamine Oxidase / pharmacology
  • Monoamine Oxidase / therapeutic use*
  • Oxidative Stress / drug effects
  • Protective Agents / pharmacology
  • Protective Agents / therapeutic use*
  • Rats, Sprague-Dawley
  • Triiodobenzoic Acids / toxicity

Substances

  • Contrast Media
  • Protective Agents
  • Triiodobenzoic Acids
  • Creatinine
  • Monoamine Oxidase
  • renalase
  • ioversol

Grants and funding

This work was sponsored by the National Natural Science Foundation of China (81100528) (http://www.nsfc.gov.cn/), the New-100 Talent Plan of Shanghai Jiao Tong University School of Medicine, Shanghai talents development fund and Shanghai Science and Technology Commission (124119a4900)(http://www.stcsm.gov.cn/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.