(1)H, (15)N, (13)C resonance assignment of human osteopontin

Biomol NMR Assign. 2015 Oct;9(2):289-92. doi: 10.1007/s12104-014-9594-7. Epub 2015 Jan 24.

Abstract

Osteopontin (OPN) is a 33.7 kDa intrinsically disordered protein and a member of the SIBLING family of proteins. OPN is bearing a signal peptide for secretion into the extracellular space, where it exerts its main physiological function, the control of calcium biomineralization. It is often involved in tumorigenic processes influencing proliferation, migration and survival, as well as the adhesive properties of cancer cells via CD44 and integrin signaling pathways. Here we report the nearly complete NMR chemical shift assignment of recombinant human osteopontin.

Keywords: Biomineralization; Extracellular matrix; Intrinsically disordered protein; Osteopontin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbon-13 Magnetic Resonance Spectroscopy*
  • Humans
  • Nitrogen Isotopes
  • Nuclear Magnetic Resonance, Biomolecular
  • Osteopontin / chemistry*
  • Protein Structure, Secondary
  • Proton Magnetic Resonance Spectroscopy*

Substances

  • Nitrogen Isotopes
  • Osteopontin