Abstract
The effect of intracerebroventricular injections of pertussis toxin were investigated on the neuropeptide Y-induced modulation of alpha 2-adrenoceptor binding in membranes from the dorsomedial medulla oblongata of the rat. Concentration-response experiments showed that neuropeptide Y reduced the binding affinity of the alpha 2-agonist, p-[3H]aminoclonidine, with a maximal effect of 30% at 3-30 nM. Pertussis toxin treatment (10 micrograms, 24 h) counteracted this modulation, without reducing the binding of neuropeptide Y to its own receptor. The results indicate that pertussis toxin-sensitive G-proteins are essential for the mediation of the intramembrane interaction between neuropeptide Y receptors and alpha 2-adrenoceptors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Diphosphate Ribose / metabolism
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Animals
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Autoradiography
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In Vitro Techniques
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Injections, Intraventricular
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Kinetics
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Male
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Medulla Oblongata / drug effects
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Medulla Oblongata / metabolism
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Membranes / drug effects
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Membranes / metabolism
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Pertussis Toxin*
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Rats
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Rats, Inbred Strains
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Receptors, Adrenergic, alpha / drug effects
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Receptors, Adrenergic, alpha / metabolism*
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Receptors, Neuropeptide Y
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Receptors, Neurotransmitter / metabolism*
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Virulence Factors, Bordetella / administration & dosage
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Virulence Factors, Bordetella / pharmacology*
Substances
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Receptors, Adrenergic, alpha
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Receptors, Neuropeptide Y
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Receptors, Neurotransmitter
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Virulence Factors, Bordetella
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Adenosine Diphosphate Ribose
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Pertussis Toxin