How FlAsH got its sparkle: historical recollections of the biarsenical-tetracysteine tag

Methods Mol Biol. 2015:1266:1-6. doi: 10.1007/978-1-4939-2272-7_1.

Abstract

The biarsenical-tetracysteine tagging system was the first of (and inspiration for) the now numerous methods for site-specifically labeling proteins in living cells with small molecules such as fluorophores. This historical recollection describes its conception and the trial-and-error chemical development required to become a versatile technique.

Publication types

  • Historical Article

MeSH terms

  • Arsenicals / chemistry*
  • Cysteine / chemistry*
  • History, 20th Century
  • Recombinant Fusion Proteins / chemistry*
  • Staining and Labeling / history

Substances

  • Arsenicals
  • Recombinant Fusion Proteins
  • Cysteine