The effect of acute alcohol intoxication on gut wall integrity in healthy male volunteers; a randomized controlled trial

Alcohol. 2015 Feb;49(1):65-70. doi: 10.1016/j.alcohol.2014.09.033. Epub 2014 Dec 9.

Abstract

The aim of the study is to determine the effect of acute alcohol consumption on enterocytes. Chronic alcohol consumption has been known to induce a decrease in gut wall integrity in actively drinking alcoholics and patients with alcohol-induced liver disease. Data on the extent of the damage induced by acute alcohol consumption in healthy human beings is scarce. Studies show that heavy incidental alcohol consumption is a growing problem in modern society. Data on this matter may provide insights into the consequences of this behavior for healthy individuals. In a randomized clinical trial in crossover design, 15 healthy volunteers consumed water one day and alcohol the other. One blood sample was collected pre-consumption, five every hour post-consumption, and one after 24 h. Intestinal fatty acid binding protein (I-FABP) was used as a marker for enterocyte damage. Liver fatty acid binding protein (L-FABP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and gamma-glutamyl transferase (GGT) were used as markers for hepatocyte damage. Lipopolysaccharide binding protein (LBP) and soluble CD14 (sCD14) were used as a measure of translocation. Interleukin-6 (IL-6) was used to assess the acute inflammatory response to endotoxemia. Alcohol consumption caused a significant increase in serum I- and L-FABP levels, compared to water consumption. Levels increased directly post-consumption and decreased to normal levels within 4 h. LBP, sCD14, and IL-6 levels were not significantly higher in the alcohol group. Moderate acute alcohol consumption immediately damages the enterocyte but does not seem to cause endotoxemia.

Keywords: Alcohol; Endotoxemia; Fatty acid binding protein; Gut wall integrity.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Alcoholic Intoxication / metabolism*
  • Cross-Over Studies
  • Ethanol / administration & dosage
  • Ethanol / toxicity*
  • Fatty Acid-Binding Proteins / blood*
  • Gastrointestinal Tract / drug effects*
  • Gastrointestinal Tract / metabolism*
  • Healthy Volunteers
  • Humans
  • Male
  • Single-Blind Method
  • Time Factors
  • Young Adult

Substances

  • FABP1 protein, human
  • Fatty Acid-Binding Proteins
  • Ethanol