Eradication of adenovirus E1-induced tumors by E1A-specific cytotoxic T lymphocytes

Cell. 1989 Nov 17;59(4):603-14. doi: 10.1016/0092-8674(89)90006-8.

Abstract

Cytotoxic T lymphocyte (CTL) clones against adenovirus type 5 (Ad5) early region 1 (E1)-transformed cells were generated in C57BL/6 (B6) mice. A defined peptide encoded by Ad5 E1A is the target structure for H-2Db-restricted CTLs. Upon intravenous injection into B6 nude mice bearing Ad5 E1-induced tumors, these CTLs, if combined with recombinant IL-2, destroy subcutaneous tumor masses up to 10 cm3. The in vivo action of CTLs is highly specific, and long-term "memory" persists in treated nude mice months after tumor regression. Our data show an important role for CTLs directed against a viral nuclear oncogene product in tumor eradication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae Infections / immunology*
  • Adenoviridae Infections / therapy
  • Adenovirus Early Proteins
  • Amino Acid Sequence
  • Animals
  • Antigens, Viral, Tumor / immunology*
  • Cells, Cultured
  • Clone Cells
  • Cytotoxicity, Immunologic*
  • DNA Probes
  • Immunotherapy*
  • Killer Cells, Natural / immunology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Inbred Strains
  • Mice, Nude
  • Molecular Sequence Data
  • Neoplasms, Experimental / immunology*
  • Neoplasms, Experimental / therapy
  • Neoplasms, Experimental / ultrastructure
  • Oncogene Proteins, Viral / genetics
  • Oncogene Proteins, Viral / immunology*
  • Peptide Fragments / chemical synthesis
  • T-Lymphocytes, Cytotoxic / immunology*
  • T-Lymphocytes, Cytotoxic / transplantation
  • T-Lymphocytes, Cytotoxic / ultrastructure

Substances

  • Adenovirus Early Proteins
  • Antigens, Viral, Tumor
  • DNA Probes
  • Oncogene Proteins, Viral
  • Peptide Fragments