Improved serotype-specific dengue virus detection in Trinidad and Tobago using a multiplex, real-time RT-PCR

Diagn Microbiol Infect Dis. 2015 Feb;81(2):105-6. doi: 10.1016/j.diagmicrobio.2014.10.001. Epub 2014 Oct 7.

Abstract

Dengue virus (DENV) transmission occurs throughout the Caribbean, though laboratory confirmation and epidemiologic surveillance are limited by the availability of serotype-specific molecular diagnostics. In this study, we show that a serotype-specific DENV multiplex, real-time reverse transcriptase-PCR (RT-PCR) detected DENV RNA in significantly more samples (82/182) than a reference hemi-nested RT-PCR (57/182; P=0.01).

Keywords: Dengue; Molecular; Pathology.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Child
  • Child, Preschool
  • Dengue / diagnosis*
  • Dengue / virology*
  • Dengue Virus / classification*
  • Dengue Virus / genetics*
  • Female
  • Humans
  • Male
  • Middle Aged
  • Molecular Diagnostic Techniques / methods*
  • Multiplex Polymerase Chain Reaction / methods*
  • Real-Time Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Serogroup
  • Trinidad and Tobago
  • Young Adult