Effects of the trace amine associated receptor 1 agonist RO5263397 on abuse-related behavioral indices of methamphetamine in rats

Int J Neuropsychopharmacol. 2014 Oct 31;18(4):pyu060. doi: 10.1093/ijnp/pyu060.

Abstract

Background: Methamphetamine is a major drug of abuse with no effective pharmacotherapy available. Trace amine associated receptor 1 is implicated in cocaine addiction and represents a potential therapeutic target. However, the effects of trace amine associated receptor 1 agonists on addiction-related behavioral effects of methamphetamine are unknown.

Methods: This study examined the effects of a trace amine associated receptor 1 agonist RO5263397 on methamphetamine-induced behavioral sensitization, methamphetamine self-administration, cue- and methamphetamine-induced reinstatement of drug seeking, and cue-induced reinstatement of sucrose-seeking behaviors in rats. Male Sprague-Dawley rats were used to examine the effects of methamphetamine alone and in combination with the trace amine associated receptor 1 agonist RO5263397 (3.2-10mg/kg).

Results: RO5263397 dose-dependently attenuated the expression of behavioral sensitization to methamphetamine, reduced methamphetamine self-administration, and decreased both cue- and a priming dose of methamphetamine-induced reinstatement of drug-seeking behaviors. However, RO5263397 did not alter cue-induced reinstatement of sucrose-seeking behavior.

Conclusions: Taken together, trace amine associated receptor 1 agonists attenuate some abuse-related behavioral effects of methamphetamine, strongly suggesting that drugs activating trace amine associated receptor 1 may be potentially useful for the treatment of methamphetamine addiction and warrant further studies.

Keywords: TAAR 1; methamphetamine; rats; reinstatement; self-administration.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Akathisia, Drug-Induced / drug therapy
  • Akathisia, Drug-Induced / physiopathology
  • Amphetamine-Related Disorders / drug therapy*
  • Amphetamine-Related Disorders / physiopathology
  • Animals
  • Behavior, Addictive / drug therapy
  • Behavior, Addictive / physiopathology
  • Central Nervous System Stimulants / administration & dosage*
  • Cues
  • Dietary Sucrose / administration & dosage
  • Dose-Response Relationship, Drug
  • Drug-Seeking Behavior / drug effects
  • Drug-Seeking Behavior / physiology
  • Male
  • Methamphetamine / administration & dosage*
  • Oxazoles / pharmacology*
  • Rats, Sprague-Dawley
  • Receptors, G-Protein-Coupled / agonists*
  • Receptors, G-Protein-Coupled / metabolism
  • Self Administration

Substances

  • 4-(3-fluoro-2-methylphenyl)-4,5-dihydrooxazol-2-ylamine
  • Central Nervous System Stimulants
  • Dietary Sucrose
  • Oxazoles
  • Receptors, G-Protein-Coupled
  • Methamphetamine
  • Trace amine-associated receptor 1