Abstract
Metabolic modifications during the developmental period can extend longevity. We found that malic enzyme (Men) overexpression during the larval period lengthened the lifespan of Drosophila. Men overexpression by S106-GeneSwitch-Gal4 driver increased pyruvate content and NADPH/NADP(+) ratio but reduced triglyceride, glycogen, and ATP levels in the larvae. ROS levels increased unexpectedly in Men-overexpressing larvae. Interestingly, adults exposed to larval Men-overexpression maintained ROS tolerance with enhanced expression levels of glutathione-S-transferase D2 and thioredoxin-2. Our results suggest that metabolic changes mediated by Men during development might be related to the control of ROS tolerance and the longevity of Drosophila.
Keywords:
Drosophila; Longevity; Malic enzyme; Pyruvate; ROS.
Copyright © 2014 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / metabolism
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Animals
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Drosophila Proteins / biosynthesis*
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Drosophila Proteins / genetics
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Drosophila melanogaster / enzymology
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Drosophila melanogaster / genetics
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Drosophila melanogaster / growth & development*
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Glutathione Transferase / metabolism
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Glycogen / metabolism
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Larva / enzymology
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Larva / genetics
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Larva / growth & development
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Longevity / genetics
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Longevity / physiology*
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Malate Dehydrogenase / biosynthesis*
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Malate Dehydrogenase / genetics
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NADP / metabolism
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Pyruvic Acid / metabolism
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Reactive Oxygen Species / metabolism
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Thioredoxins / metabolism
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Triglycerides / metabolism
Substances
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Drosophila Proteins
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Reactive Oxygen Species
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Triglycerides
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Thioredoxins
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NADP
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Pyruvic Acid
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Adenosine Triphosphate
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Glycogen
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Malate Dehydrogenase
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malate dehydrogenase (decarboxylating)
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malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)
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Glutathione Transferase