Network-based gene expression analysis of vascular wall of juvenile Moyamoya disease

Childs Nerv Syst. 2015 Mar;31(3):399-404. doi: 10.1007/s00381-014-2605-2. Epub 2014 Dec 12.

Abstract

Subjects: Little is known about the pathology and pathogenesis of the Moyamoya disease (MMD). To better understand the molecular processes involved in MMD gene expression analysis of Moyamoya artery tissue to a control artery, this study was conducted.

Methods: Tissue samples of two MMD and two non-MMD were profiled using oligonucleotide microarrays. Gene ontology classification of the differentially expressed genes was analyzed, and regulatory functional networks and pathways were identified with a network-based computational pathway analysis tool. Analysis of MMD and control tissue revealed 104 differentially expressed genes.

Results: The two major significantly associated gene ontology terms was cellular development and cellular movement. Further network-based analysis showed significant interaction between RNF213 downstream gene networks and the top 3 score gene networks of MMD. Three major nodes of this network were evident in the merged network and were showing interactions with downstream network of RNF213.

Conclusions: Our results demonstrate that cellular development and cellular movement in MMD are the key role of mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Cerebral Angiography
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation / physiology*
  • Gene Ontology
  • Gene Regulatory Networks / genetics*
  • Humans
  • Male
  • Moyamoya Disease / genetics
  • Moyamoya Disease / physiopathology*
  • Oligonucleotide Array Sequence Analysis