Platelet-derived-growth-factor-stimulated heterogeneous polyphosphoinositide metabolism and phosphate uptake in C3H fibroblasts

H Holmsen; R Male; S Rongved; N Langeland; J Lillehaug

doi:10.1042/bj2600589

Platelet-derived-growth-factor-stimulated heterogeneous polyphosphoinositide metabolism and phosphate uptake in C3H fibroblasts

Biochem J. 1989 Jun 1;260(2):589-92. doi: 10.1042/bj2600589.

Authors

H Holmsen¹, R Male, S Rongved, N Langeland, J Lillehaug

Affiliation

¹ Department of Biochemistry, University of Bergen, Norway.

Abstract

Pig platelet-derived growth factor (PDGF) increased the rate of [32P]Pi uptake by murine fibroblasts, resulting in a 3-9-fold elevation of the specific radioactivity of ATP, PtdInsP, PtdInsP2, PtdIns and phosphatidic acid. The specific radioactivity was 10-60-fold higher in ATP than in the four phospholipids. These substances are therefore not in metabolic equilibrium, which complicates determination of inositol phospholipid turnover.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphate / metabolism
Animals
Fibroblasts / drug effects
Fibroblasts / metabolism
Mice
Phosphates / metabolism*
Phosphatidic Acids / metabolism
Phosphatidylinositol Phosphates
Phosphatidylinositols / metabolism*
Platelet-Derived Growth Factor / pharmacology*

Substances

Phosphates
Phosphatidic Acids
Phosphatidylinositol Phosphates
Phosphatidylinositols
Platelet-Derived Growth Factor
Adenosine Triphosphate