Properties of vasoactive-intestinal-peptide receptors and beta-adrenoceptors in the murine radiation leukemia-virus-induced lymphoma cell line BL/VL3

Eur J Biochem. 1989 Aug 1;183(2):263-7. doi: 10.1111/j.1432-1033.1989.tb14922.x.

Abstract

1. Based on radioligand binding and adenylate cyclase activation, functional receptors to vasoactive intestinal peptide(VIP)/helodermin, were shown to coexist with beta 2-adrenoceptors and prostaglandin receptors in membranes from a cultured cloned BL/VL3 cell line of murine T-cell lymphoma induced by a radiation leukemia virus. 2. The relative potency of VIP-related peptides to stimulate adenylate cyclase activity was: helodermin greater than VIP greater than peptide histidine isoleucinamide. Five VIP analogs inhibited 125I-iodo-VIP binding and stimulated adenylate cyclase activity, their decreasing order of potency being: VIP greater than [D-Asp3]VIP greater than [D-Ser2]VIP greater than [D-Ala4]VIP = [D-His1]VIP = [D-Phe2]VIP. [D-Phe2]VIP acted as a partial agonist (with an intrinsic activity of 0.1 as compared to that of VIP = 1.0) and competitively inhibited helodermin- and VIP-stimulated adenylate cyclase activity with a similar Ki (0.07-0.10 microM). These data suggest the existence, in this murine T-cell lymphoma, of VIP receptors of the 'helodermin-preferring' subtype that are coupled to adenylate cyclase.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenylyl Cyclases / metabolism
  • Alprostadil / pharmacology
  • Animals
  • Catecholamines / pharmacology
  • Cell Membrane / metabolism
  • Enzyme Activation / drug effects
  • Intercellular Signaling Peptides and Proteins
  • Leukemia, Radiation-Induced*
  • Lymphoma / etiology
  • Lymphoma / metabolism*
  • Mice
  • Peptide PHI / pharmacology
  • Peptides / pharmacology
  • Receptors, Adrenergic, beta / physiology*
  • Receptors, Gastrointestinal Hormone / physiology*
  • Receptors, Prostaglandin / physiology
  • Receptors, Vasoactive Intestinal Peptide
  • Retroviridae*
  • T-Lymphocytes
  • Tumor Cells, Cultured
  • Vasoactive Intestinal Peptide / analogs & derivatives
  • Vasoactive Intestinal Peptide / metabolism
  • Vasoactive Intestinal Peptide / pharmacology

Substances

  • Catecholamines
  • Intercellular Signaling Peptides and Proteins
  • Peptide PHI
  • Peptides
  • Receptors, Adrenergic, beta
  • Receptors, Gastrointestinal Hormone
  • Receptors, Prostaglandin
  • Receptors, Vasoactive Intestinal Peptide
  • Vasoactive Intestinal Peptide
  • heliodermin
  • Adenylyl Cyclases
  • Alprostadil