Hydrazone conjugates of polymers with doxorubicin represent a very promising tool for cancer chemotherapy. However, these conjugates are very difficult to radiolabel with iodine radionuclides, which possess otherwise very advantageous nuclear properties to, e.g., follow biodistribution. In this study, we developed a robust protocol for the high-yield radioiodination of hydrazone-type drug delivery systems with doxorubicin. In particular, it is crucial that the polymer radioiodination step be performed before the deprotection of the hydrazide and doxorubicin binding.
Keywords: Doxorubicin; Hydrazone; Polymer conjugate; Radioiodination; Radiolabeling; poly[N-(2-hydroxypropyl)methacrylamide].
Copyright © 2014 Elsevier Ltd. All rights reserved.