Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins

Methods Mol Biol. 2015:1258:209-22. doi: 10.1007/978-1-4939-2205-5_11.

Abstract

Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable lab-scale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • HEK293 Cells / metabolism*
  • Humans
  • Mammals / genetics*
  • Mammals / metabolism
  • Recombinant Proteins / genetics*
  • Recombinant Proteins / metabolism
  • Transfection / methods

Substances

  • Recombinant Proteins