Surface profiles of live colorectal cancer cells and tumor infiltrating lymphocytes from surgical samples correspond to prognostic categories

Jerry Zhou; Larissa Belov; Pierre Chapuis; Charles Chan; Nicola Armstrong; Kimberley L Kaufman; Michael J Solomon; Stephen J Clarke; Richard I Christopherson

doi:10.1016/j.jim.2014.11.001

Surface profiles of live colorectal cancer cells and tumor infiltrating lymphocytes from surgical samples correspond to prognostic categories

J Immunol Methods. 2015 Jan:416:59-68. doi: 10.1016/j.jim.2014.11.001. Epub 2014 Nov 9.

Authors

Jerry Zhou¹, Larissa Belov¹, Pierre Chapuis², Charles Chan³, Nicola Armstrong⁴, Kimberley L Kaufman¹, Michael J Solomon⁵, Stephen J Clarke⁶, Richard I Christopherson⁷

Affiliations

¹ School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia.
² Discipline of Surgery, Sydney Clinical School, University of Sydney, Sydney, NSW 2006, Australia; Department of Colorectal Surgery, Concord Hospital, Concord West, NSW 2139, Australia.
³ Department of Anatomical Pathology, Concord Hospital, Concord West, NSW 2139, Australia; Discipline of Pathology, Concord Clinical School, University of Sydney, Sydney, NSW 2006, Australia.
⁴ School of Mathematics and Statistics, University of Sydney, Sydney, NSW 2006, Australia.
⁵ Discipline of Surgery, Sydney Clinical School, University of Sydney, Sydney, NSW 2006, Australia; Discipline of Surgery, Royal Prince Alfred Hospital, Camperdown, NSW 2050, Australia.
⁶ Bill Walsh Cancer Research Laboratories, Royal North Shore Hospital, St Leonards, NSW 2065, Australia.
⁷ School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia. Electronic address: richard.christopherson@sydney.edu.au.

PMID: 25445327
DOI: 10.1016/j.jim.2014.11.001

Abstract

Extensive surface profiles of colorectal cancer (CRC) cells and tumor infiltrating lymphocytes (TIL) have been obtained from 45 surgical resection samples. Live cells were captured on an antibody microarray and stained with fluorescently-labeled antibodies. Minimal panels of 11 CRC antigens (CD13, CD24, CD26, CD49d, CD138, CD166, CA-125, CA19-9, EGFR, Galectin-4 and HLA-DR) and 11 T-cell antigens (CD10, CD11b, CD11c, CD25, CD31, CD95, CD151, CD181, Galectin-4, CA19-9, TSP-1) provide signatures for relapse and survival. Hierarchical clustering of profiles from CRC cells and TIL identified groups of patients for survival, systemic relapse and death. The groups from CRC and TIL profiles for systemic relapse showed 79.2% concordance, enabling prediction of relapse after surgery. The results demonstrate communication between CRC cells and TIL.

Keywords: Antibody microarray; Biomarkers; Colorectal cancer; Disease signature; Tumor infiltrating T-lymphocytes.

MeSH terms

Aged
Antigens, CD / immunology
Colorectal Neoplasms / immunology*
Colorectal Neoplasms / pathology*
Epitopes, T-Lymphocyte / immunology
Female
HLA-DR Antigens / immunology
Humans
Lymphocytes, Tumor-Infiltrating / immunology*
Lymphocytes, Tumor-Infiltrating / pathology*
Male
Neoplasm Recurrence, Local / immunology
Neoplasm Recurrence, Local / pathology
Prognosis

Substances

Antigens, CD
Epitopes, T-Lymphocyte
HLA-DR Antigens