Abstract
A novel micelle/precursor co-templating assembly strategy is successfully developed to synthesize large-pore ultrasmall mesoporous organosilica nanoparticles (MONs). Furthermore, elaborately designed MONs with a cell-penetrating peptide (TAT) (MONs-PTAT) are constructed for highly efficient intranuclear gene delivery. They exhibit a high loading capacity, improved protection for the loaded gene, and enhanced transfection efficiencies of EGFP plasmid (pEGFP).
Keywords:
gene delivery; large mesopores; mesoporous organosilicas; nuclear-targeted.
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biocompatible Materials / chemistry
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Cell Survival
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Gene Products, tat / genetics
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Gene Transfer Techniques*
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Genetic Therapy / methods
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HeLa Cells
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Humans
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Hydrophobic and Hydrophilic Interactions
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Magnetic Resonance Spectroscopy
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Micelles*
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Microscopy, Electron, Scanning
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Microscopy, Electron, Transmission
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Nanoparticles / chemistry*
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Particle Size
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Plasmids / genetics
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Porosity
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Silicon Dioxide / chemistry
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Spectroscopy, Fourier Transform Infrared
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Spectrum Analysis, Raman
Substances
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Biocompatible Materials
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Gene Products, tat
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Micelles
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Silicon Dioxide