Scalable low-cost fabrication of disposable paper sensors for DNA detection

ACS Appl Mater Interfaces. 2014 Dec 24;6(24):22751-60. doi: 10.1021/am5069003. Epub 2014 Dec 5.

Abstract

Controlled integration of features that enhance the analytical performance of a sensor chip is a challenging task in the development of paper sensors. A critical issue in the fabrication of low-cost biosensor chips is the activation of the device surface in a reliable and controllable manner compatible with large-scale production. Here, we report stable, well-adherent, and repeatable site-selective deposition of bioreactive amine functionalities and biorepellant polyethylene glycol-like (PEG) functionalities on paper sensors by aerosol-assisted, atmospheric-pressure, plasma-enhanced chemical vapor deposition. This approach requires only 20 s of deposition time, compared to previous reports on cellulose functionalization, which takes hours. A detailed analysis of the near-edge X-ray absorption fine structure (NEXAFS) and its sensitivity to the local electronic structure of the carbon and nitrogen functionalities. σ*, π*, and Rydberg transitions in C and N K-edges are presented. Application of the plasma-processed paper sensors in DNA detection is also demonstrated.

Keywords: DNA detection; NEXAFS; X-ray absorption; cellulose functionalization; paper sensors.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • Biosensing Techniques / instrumentation*
  • DNA / analysis
  • DNA / genetics*
  • Disposable Equipment*
  • Equipment Design
  • Equipment Failure Analysis
  • Molecular Sequence Data
  • Oligonucleotide Array Sequence Analysis / instrumentation*
  • Paper*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Systems Integration

Substances

  • DNA