Comparison of chromogenic media for recovery of carbapenemase-producing enterobacteriaceae (CPE) and evaluation of CPE prevalence at a tertiary care academic medical center

Morgan A Pence; Tiffany Hink; Carey-Ann D Burnham

doi:10.1128/JCM.03208-14

Comparison of chromogenic media for recovery of carbapenemase-producing enterobacteriaceae (CPE) and evaluation of CPE prevalence at a tertiary care academic medical center

J Clin Microbiol. 2015 Feb;53(2):663-6. doi: 10.1128/JCM.03208-14. Epub 2014 Nov 19.

Authors

Morgan A Pence¹, Tiffany Hink², Carey-Ann D Burnham³

Affiliations

¹ Department of Pathology and Immunology, Division of Laboratory and Genomic Medicine, Washington University School of Medicine, St. Louis, Missouri, USA.
² Department of Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, Missouri, USA.
³ Department of Pathology and Immunology, Division of Laboratory and Genomic Medicine, Washington University School of Medicine, St. Louis, Missouri, USA cburnham@path.wustl.edu.

Abstract

We evaluated the performance characteristics of chromID CARBA and HardyCHROM Carbapenemase for the detection of carbapenemase-producing Enterobacteriaceae (CPE). A CPE prevalence study was conducted using chromID CARBA; this demonstrated that in low-prevalence settings, CPE screening agars may lack specificity, and confirmation of putative isolates is necessary.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Academic Medical Centers
Bacterial Proteins / metabolism*
Chromogenic Compounds / metabolism*
Culture Media / chemistry*
Enterobacteriaceae / enzymology*
Enterobacteriaceae / growth & development
Enterobacteriaceae / isolation & purification*
Enterobacteriaceae Infections / epidemiology*
Enterobacteriaceae Infections / microbiology
Humans
Prevalence
Sensitivity and Specificity
Tertiary Healthcare
beta-Lactamases / metabolism*

Substances

Bacterial Proteins
Chromogenic Compounds
Culture Media
beta-Lactamases
carbapenemase