Hybrid ZnO nanorod-polymer brush hierarchically nanostructured substrate for sensitive antibody microarrays

Adv Mater. 2015 Jan 7;27(1):181-5. doi: 10.1002/adma.201403712. Epub 2014 Nov 3.

Abstract

A hierarchically nanostructured organic-inorganic hybrid substrate comprising randomly oriented ZnO nanorods on glass slide with coaxially tethered dense polymer brush, POEGMA-co-GMA is reported for highly sensitive antibody microassay, achieving excellent detection specificity, and superior detection limit of as low as 100 fg mL(-1) for biomarkers in human serum within a 1 h assay time.

Keywords: Antibody Microarray; Cancer Biomarker; Nanostructured Substrate; Polymer Brush; ZnO Nanorod.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / analysis*
  • Biomarkers / analysis
  • Blood Chemical Analysis / instrumentation
  • Blood Chemical Analysis / methods
  • Epoxy Compounds / chemistry
  • Fluorescence
  • Glass / chemistry
  • Humans
  • Immunoassay / instrumentation
  • Immunoassay / methods
  • Methacrylates / chemistry
  • Microarray Analysis / instrumentation*
  • Microarray Analysis / methods
  • Microscopy, Electron, Scanning
  • Microscopy, Electron, Transmission
  • Nanotubes / chemistry*
  • Polyethylene Glycols / chemistry
  • Polymers / chemistry*
  • Polymethacrylic Acids
  • Sensitivity and Specificity
  • Zinc Oxide / chemistry*

Substances

  • Antibodies
  • Biomarkers
  • Epoxy Compounds
  • Methacrylates
  • Polymers
  • Polymethacrylic Acids
  • poly(poly(ethylene glycol)methacrylate)
  • Polyethylene Glycols
  • glycidyl methacrylate
  • Zinc Oxide