Sensitizing B- and T- cell Lymphoma Cells to Paclitaxel/Abraxane-Induced Death by AS101 via Inhibition of the VLA-4-IL10-Survivin Axis

Mol Cancer Res. 2015 Mar;13(3):411-22. doi: 10.1158/1541-7786.MCR-14-0459. Epub 2014 Oct 28.

Abstract

Cancer cell resistance to chemotherapy is a major concern in clinical oncology, resulting in increased tumor growth and decreased patient survival. Manipulation of apoptosis has emerged as a new therapeutic strategy to eliminate cancer cells. The focus of this study resides within a novel approach to target survivin, an integrator of both cell death and mitosis. This protein plays a pivotal role in the resistance of tumors to chemotherapy, especially to paclitaxel. The data herein demonstrate an indirect repression of survivin in both B- and T-cell lymphoma and human NHL by the nontoxic tellurium compound, AS101 [ammonium trichloro(dioxoethylene-o,o')tellurate], via inhibition of tumor autocrine IL10-STAT3-Survivin signaling. As a result of survivin abrogation, sensitization of lymphomas to paclitaxel or to Abraxane, the new albumin-stabilized nanoparticle formulation of paclitaxel, occurs both in vitro and in vivo. Importantly, inhibition of lymphoma cell IL10 secretion is mediated by inactivation of the VLA-4 integrin, recently shown to be an important target of AS101. This activity is followed by inhibition of the PI3K-AKT axis that mediates IL10 suppression. Because a wide variety of lymphomas and other tumor types express VLA-4 and secrete IL10 in an autocrine manner, inhibition of survivin with a small nontoxic agent has vast clinical significance in modulating chemosensitivity in many tumor types.

Implications: Combination therapy with AS101 and paclitaxel has novel therapeutic potential targeting deregulated active pathways in lymphoma, overcoming endogenous resistance to apoptosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / administration & dosage*
  • Antineoplastic Agents / pharmacology
  • Antineoplastic Combined Chemotherapy Protocols / administration & dosage
  • Antineoplastic Combined Chemotherapy Protocols / pharmacology
  • Apoptosis
  • Cell Line, Tumor
  • Drug Resistance, Neoplasm / drug effects
  • Drug Synergism
  • Ethylenes / administration & dosage*
  • Ethylenes / pharmacology
  • Humans
  • Inhibitor of Apoptosis Proteins / metabolism
  • Integrin alpha4beta1 / metabolism
  • Interleukin-10 / metabolism
  • Lymphoma, B-Cell / drug therapy*
  • Lymphoma, B-Cell / metabolism
  • Lymphoma, T-Cell / drug therapy*
  • Lymphoma, T-Cell / metabolism
  • Mice
  • Neoplasm Transplantation
  • Paclitaxel / administration & dosage*
  • Paclitaxel / pharmacology
  • Repressor Proteins / metabolism
  • Signal Transduction / drug effects*
  • Survivin

Substances

  • Antineoplastic Agents
  • Birc5 protein, mouse
  • Ethylenes
  • IL10 protein, mouse
  • Inhibitor of Apoptosis Proteins
  • Integrin alpha4beta1
  • Repressor Proteins
  • Survivin
  • Interleukin-10
  • ammonium trichloro(dioxoethylene-O,O'-)tellurate
  • Paclitaxel