Bacterial metabarcoding by 16S rRNA gene ion torrent amplicon sequencing

Methods Mol Biol. 2015:1231:77-90. doi: 10.1007/978-1-4939-1720-4_5.

Abstract

Ion Torrent is a next generation sequencing technology based on the detection of hydrogen ions produced during DNA chain elongation; this technology allows analyzing and characterizing genomes, genes, and species. Here, we describe an Ion Torrent procedure applied to the metagenomic analysis of 16S rRNA gene amplicons to study the bacterial diversity in food and environmental samples.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / genetics*
  • Chromosome Mapping
  • DNA Barcoding, Taxonomic / methods
  • DNA Barcoding, Taxonomic / statistics & numerical data*
  • DNA Primers / chemical synthesis
  • DNA Primers / chemistry
  • Electrophoresis, Agar Gel
  • Genes, Bacterial*
  • Genome, Bacterial*
  • Genomic Library
  • High-Throughput Nucleotide Sequencing / instrumentation
  • High-Throughput Nucleotide Sequencing / methods*
  • Metagenomics
  • Molecular Sequence Annotation
  • Polymerase Chain Reaction / methods
  • Protons
  • RNA, Ribosomal, 16S / genetics*
  • Sequence Analysis, DNA / statistics & numerical data

Substances

  • DNA Primers
  • Protons
  • RNA, Ribosomal, 16S