Abstract
We have used polymerase chain reaction to accelerate our analysis of recombinant lambda-cDNA clones. We have amplified the inserts of lambda gt10 or lambda gt11 recombinants starting with bacteriophage in cored plaques or isolated DNA. The amplifications made with simple or complex oligonucleotide primers have allowed convenient sizing, subcloning and translation of the phage inserts into protein.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Bacteriophage lambda / genetics
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Base Sequence
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Biotechnology
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Cloning, Molecular / methods*
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DNA* / genetics
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DNA, Recombinant
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Molecular Sequence Data
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Nucleic Acid Amplification Techniques*
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Polymerase Chain Reaction / methods*
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Viral Proteins / genetics
Substances
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DNA, Recombinant
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Viral Proteins
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DNA