In vitro genotoxicity of mycotoxins ochratoxin A and fumonisin B(1) could be prevented by sodium copper chlorophyllin--implication to their genotoxic mechanism

Food Chem. 2015 Mar 1:170:455-62. doi: 10.1016/j.foodchem.2014.08.036. Epub 2014 Aug 19.

Abstract

The aim of this study was to investigate the possible protective effect of sodium copper chlorophyllin (CHL) against cytotoxicity and DNA damage induced by mycotoxins ochratoxin A (OTA) and fumonisin B1 (FB1). CHL (0.1-100 μg/ml) alone had no impact on cell viability and genome damage in the primary human peripheral blood lymphocytes (HPBLs) and exhibited free radical scavenging activity in the DPPH assay. Both mycotoxins, OTA (4 μmol/l) and FB1 (20 μg/ml), induced DNA damage in HPBLs already after 1 h exposure. When the HPBLs were co-exposed to CHL (10 and 100 μg/ml) and OTA (4 μmol/l) or FB1 (20 μg/ml) for 1 h, CHL protected against cell and DNA damage induced by both mycotoxins, implying that OTA and FB1 cytogenotoxicity mechanisms function at least partially through oxidative stress. Therefore, CHL could be a perfect candidate for possible use as an antioxidant.

Keywords: Chlorophyllin; DNA damage; Human lymphocytes; Mycotoxins; Oxidative stress; Protective effect.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Cell Survival / drug effects
  • Cells, Cultured
  • Chlorophyllides / chemistry
  • Chlorophyllides / pharmacology*
  • DNA Damage / drug effects*
  • Female
  • Free Radical Scavengers / chemistry
  • Fumonisins / toxicity*
  • Humans
  • Leukocytes, Mononuclear / cytology
  • Leukocytes, Mononuclear / drug effects
  • Ochratoxins / toxicity*
  • Oxidative Stress / drug effects
  • Protective Agents / chemistry
  • Protective Agents / pharmacology*
  • Toxicity Tests

Substances

  • Chlorophyllides
  • Free Radical Scavengers
  • Fumonisins
  • Ochratoxins
  • Protective Agents
  • ochratoxin A
  • fumonisin B1
  • chlorophyllin