Wide-scale pollen banking of ornamental plants through cryopreservation

Cryo Letters. 2014 Jul-Aug;35(4):312-9.

Abstract

Background: Cryopreservation has been proved to be an efficient method for the long-term storage of pollen, based on our previous studies establishing cryo pollen banks for Camellia, Paeonia, and Prunus mume.

Objective: In the present study, we aimed to extend and verify the applicability of the cryopreservation protocol for ornamental plants.

Materials and methods: 71 species or cultivars from 19 families were cryopreserved using the established protocol.

Results: Pollen from 60 species or cultivars (85% of those experimented on) retained viability after 1 year's cryopreservation; of these, 36 (51% of the total investigated) retained a sufficiently high germination capability compared with fresh pollen to enable longer term cryopreservation.

Conclusion: This study confirms that wide-scale pollen banking of ornamental plants is feasible.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Specimen Banks / statistics & numerical data*
  • Cell Survival / physiology
  • Cryopreservation / methods*
  • Germination / physiology
  • Magnoliopsida / physiology*
  • Pollen / physiology*