RNA fluorescence in situ hybridization in cultured mammalian cells

Methods Mol Biol. 2015:1206:123-36. doi: 10.1007/978-1-4939-1369-5_11.

Abstract

It is now clear that long noncoding RNAs (lncRNAs) regulate a number of aspects of nuclear organization and gene expression. An important tool for the study of the distribution and function of lncRNAs is RNA fluorescence in situ hybridization (RNA-FISH). The protocols presented in this chapter describe this method in detail and also mention a number of critical points that must be considered when performing this technique.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • DNA Probes
  • In Situ Hybridization, Fluorescence / methods*
  • Mammals
  • RNA, Long Noncoding / analysis*

Substances

  • DNA Probes
  • RNA, Long Noncoding