Sensitive and selective amplified visual detection of cytokines based on exonuclease III-aided target recycling

Chem Commun (Camb). 2014 Nov 11;50(87):13342-5. doi: 10.1039/c4cc05701e. Epub 2014 Sep 18.

Abstract

A new Exo III assisted strand-cleavage cycle and ligand-responsive quadruplex formation strategy for amplified and label-free detection of IFN-γ was reported with a detection limit of 0.1 pM and a visual detection limit of 20 pM by the naked eye.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Exodeoxyribonucleases / metabolism*
  • Humans
  • Interferon-gamma / analysis*
  • Interferon-gamma / metabolism*
  • Ligands

Substances

  • Ligands
  • Interferon-gamma
  • Exodeoxyribonucleases
  • exodeoxyribonuclease III