In vivo administration of Fab' fragments of anti-L3T4 (GK1.5) antibody inhibits the T helper cell function of murine lymph node cells

Cell Immunol. 1989 Apr 15;120(1):75-81. doi: 10.1016/0008-8749(89)90175-5.

Abstract

The effect of intravenous injection of Fab' fragments of anti-L3T4 antibody (GK1.5 monoclonal antibody) into mice was studied. This treatment depleted L3T4+ cells from the popliteal lymph nodes of keyhole-limpet hemocyanin-primed mice. The T cells that remained were unable to provide help to antigen-specific B cells in vitro. The results obtained using Fab' fragments were comparable with those using intact anti-L3T4 antibody and demonstrate that either form of GK1.5 is a potentially useful immunosuppressive agent in mice.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibody Formation
  • Antigens, Differentiation, T-Lymphocyte / immunology*
  • Immunoglobulin Fab Fragments / immunology
  • Isoantibodies / immunology*
  • Lymph Nodes / immunology*
  • Lymphocyte Cooperation
  • Mice
  • Mice, Inbred BALB C
  • T-Lymphocytes, Helper-Inducer / immunology*

Substances

  • Antibodies, Monoclonal
  • Antigens, Differentiation, T-Lymphocyte
  • Immunoglobulin Fab Fragments
  • Isoantibodies