Polyethylenimine derivate conjugated with RGD-TAT-NLS as a novel gene vector

Biomed Mater Eng. 2014;24(6):1933-9. doi: 10.3233/BME-141002.

Abstract

To solve the contradiction between the cell toxicity and transfection efficiency of polyethylenimine (PEI) derivate in non-viral gene therapy, a novel gene vector, P123-PEI-R18 was synthesized by using biodegradable PEI derivate conjugated with trifunctional peptide RGD-TAT-NLS. The particle size of P123-PEI-R18/DNA was around 100-250 nm. The gene vector could condense DNA at the weight ratio of 2 and protect plasmid DNA from being dissolved in the blood circulation. Importantly, the complexes exhibited lower cell toxicity and higher transfection efficiency contrasted with PEI 25 kDa in vitro. P123-PEI-R18 holds high potential as a safe and efficient gene vector.

Keywords: Non-viral gene vector; P123; avß3; nuclear localization signal; polyethylenimine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Genetic Vectors / genetics
  • Humans
  • Imines / chemistry
  • Intracellular Signaling Peptides and Proteins
  • Melanoma / genetics*
  • Mice
  • Microscopy, Confocal / methods
  • Molecular Imaging / methods*
  • Nanocapsules / chemistry*
  • Nanocapsules / ultrastructure
  • Particle Size
  • Peptides / chemistry
  • Peptides / pharmacokinetics*
  • Plasmids / administration & dosage
  • Plasmids / genetics*
  • Poloxalene / chemistry
  • Polyethylenes / chemistry
  • Polymers / chemistry*
  • Transfection / methods*

Substances

  • Imines
  • Intracellular Signaling Peptides and Proteins
  • Nanocapsules
  • Peptides
  • Polyethylenes
  • Polymers
  • R18 peptide
  • poly(ethylene imine)
  • pluronic block copolymer P123
  • Poloxalene