Evaluation of Borrelia real time PCR DNA targeting OspA, FlaB and 5S-23S IGS and Borrelia 16S rRNA RT-qPCR

J Microbiol Methods. 2014 Dec:107:41-6. doi: 10.1016/j.mimet.2014.09.001. Epub 2014 Sep 16.

Abstract

Borrelia burgdorferi non-sensu lato (s.l.) strains occurred in the Netherlands. A multiplex OspA, FlaB, IGS real time PCR was compared to 16S rRNA/rDNA RT-qPCR with lower average Cycle threshold (Ct) and LOD on strain dilutions. Multiplexing increased sensitivity on CSF samples (n=74), distinguishing B. burgdorferi s.l. from non-s.l. strains.

Keywords: Borrelia; Lyme; Real time PCR.

MeSH terms

  • Adult
  • Aged
  • Antigens, Surface / genetics*
  • Bacterial Outer Membrane Proteins / genetics*
  • Bacterial Vaccines / genetics*
  • Borrelia / classification
  • Borrelia / genetics*
  • DNA, Ribosomal Spacer*
  • Female
  • Flagellin / genetics*
  • Humans
  • Lipoproteins / genetics*
  • Lyme Disease / diagnosis*
  • Lyme Disease / microbiology*
  • Male
  • Middle Aged
  • Multiplex Polymerase Chain Reaction / methods
  • RNA, Ribosomal*
  • Real-Time Polymerase Chain Reaction / methods*
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Antigens, Surface
  • Bacterial Outer Membrane Proteins
  • Bacterial Vaccines
  • DNA, Ribosomal Spacer
  • Lipoproteins
  • OspA protein
  • RNA, Ribosomal
  • Flagellin
  • flaB flagellin