CDr10b inhibits the expression of cyclooxygenase-2 and inducible nitric oxide synthase induced by lipopolysaccharide

Gyo-Jeong Gu; Se-Jin Lim; Sang-Il Ahn; Sung-Chan Lee; Young-Tae Chang; Tae Hyun Choi; Byoung Soo Kim; Yong-Bin Eom; Na Kyung Lee; Hyung-Sun Youn

doi:10.1016/j.ejphar.2014.08.036

CDr10b inhibits the expression of cyclooxygenase-2 and inducible nitric oxide synthase induced by lipopolysaccharide

Eur J Pharmacol. 2014 Nov 5:742:42-6. doi: 10.1016/j.ejphar.2014.08.036. Epub 2014 Sep 6.

Authors

Affiliations

¹ Departments of Medical Science, College of Medical Sciences, SoonChunHyang University, Chungnam, Asan 336-745, Republic of Korea.
² Department of Biomedical Laboratory Science, College of Medical Sciences, SoonChunHyang University, Chungnam, Asan 336-745, Republic of Korea.
³ Aptabio Therapeutics Inc., Kyunggi-do, Yong-in city 446-908, Republic of Korea.
⁴ Department of Chemistry and MedChem Program, Life Sciences Institute, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore; Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium (SBIC), 11 Biopolis Way, #02-02 Helios, Agency for Science, Technology and Research (A⁎STAR), Biopolis, Singapore 138667, Singapore.
⁵ Department of Imaging, Korea Institute of Radiological & Medical Sciences, Seoul 139-706, Republic of Korea.
⁶ Departments of Medical Science, College of Medical Sciences, SoonChunHyang University, Chungnam, Asan 336-745, Republic of Korea; Department of Biomedical Laboratory Science, College of Medical Sciences, SoonChunHyang University, Chungnam, Asan 336-745, Republic of Korea. Electronic address: hyoun@sch.ac.kr.

PMID: 25196213
DOI: 10.1016/j.ejphar.2014.08.036

Abstract

The pathophysiological processes of inflammation can lead to a host of diseases, such as periodontitis, atherosclerosis, rheumatoid arthritis, and even cancer. The dysregulated inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) activation play important roles in the development of certain inflammatory diseases. Here, we investigated the effects of CDr10b which is originally developed for a microglia staining probe on inflammation, by modulating NF-κB activation and iNOS and COX-2 expression induced by lipopolysaccharide (LPS) in murine macrophages. The CDr10b suppressed NF-κB activation and iNOS and COX-2 expression induced by LPS. All the results suggest that CDr10b is a promising novel agent for the treatment of inflammatory diseases.

Keywords: CDr10b; Cyclooxygenase-2; Inducible nitric oxide synthase; Inflammation; Nuclear factor-κB.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Inflammatory Agents / pharmacology
Boron Compounds / pharmacology*
Cell Line
Cyclooxygenase 2 / genetics
Cyclooxygenase 2 / metabolism*
Enzyme Activation / drug effects
Gene Expression / drug effects
Immunologic Factors / pharmacology
Inflammation / drug therapy
Inflammation / metabolism
Lipopolysaccharides / pharmacology*
Macrophages / drug effects
Macrophages / metabolism
Mice
Myeloid Differentiation Factor 88 / metabolism
NF-kappa B / metabolism
Nitric Oxide Synthase Type II / genetics
Nitric Oxide Synthase Type II / metabolism*
Signal Transduction / drug effects
Toll-Like Receptor 4 / metabolism

Substances

Anti-Inflammatory Agents
Boron Compounds
CDr10b compound
Immunologic Factors
Lipopolysaccharides
Myd88 protein, mouse
Myeloid Differentiation Factor 88
NF-kappa B
Tlr4 protein, mouse
Toll-Like Receptor 4
Nitric Oxide Synthase Type II
Nos2 protein, mouse
Ptgs2 protein, mouse
Cyclooxygenase 2