Wavelength shifting oligonucleotide probe for the detection of adenosine of a target DNA with enhanced fluorescence signal

Subhendu Sekhar Bag; Manoj K Pradhan; Suman K Das; Subhashis Jana; Raghunath Bag

doi:10.1016/j.bmcl.2014.08.031

Wavelength shifting oligonucleotide probe for the detection of adenosine of a target DNA with enhanced fluorescence signal

Bioorg Med Chem Lett. 2014 Oct 1;24(19):4678-4681. doi: 10.1016/j.bmcl.2014.08.031. Epub 2014 Aug 19.

Authors

Subhendu Sekhar Bag¹, Manoj K Pradhan², Suman K Das², Subhashis Jana², Raghunath Bag²

Affiliations

¹ Bioorganic Chemistry Laboratory, Department of Chemistry, Indian Institute of Technology, Guwahati 781039, India. Electronic address: ssbag75@iitg.ernet.in.
² Bioorganic Chemistry Laboratory, Department of Chemistry, Indian Institute of Technology, Guwahati 781039, India.

PMID: 25190467
DOI: 10.1016/j.bmcl.2014.08.031

Abstract

The modulated photophysical property of strong electronically coupled naphthyl uridine linked via a single C-C bond was explored in DNA detection via wavelength shifting and enhanced fluorescence emission by a simple 'Just-Mix & Read' strategy of homogeneous DNA detection.

Keywords: DNA detection; Fluorescence; Fluorescent oligonucleotide probe; Just Mix & Read strategy; Wavelength shift.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine / analysis*
DNA / analysis*
Fluorescence*
Oligonucleotide Probes / chemistry*
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet

Substances

Oligonucleotide Probes
DNA
Adenosine