Updating the PGMY primers and probes for improved detection of HPV68a: validation of version 2 of the PGMY-CHUV assay

J Clin Microbiol. 2014 Nov;52(11):4033-5. doi: 10.1128/JCM.01950-14. Epub 2014 Aug 20.

Abstract

HPV68a is not efficiently detected by PCR with the PGMY primers. Version 2 of the PGMY-CHUV assay (PGv2) was developed from version 1 (PGv1) to evaluate HPV68-discordant results with the Anyplex II HPV28 assay. We now report that PGv2 is significantly more sensitive than PGv1 for HPV68a and as sensitive and specific for the other HPV genotypes during a 1-year prospective validation (n = 714 samples).

Publication types

  • Evaluation Study

MeSH terms

  • DNA Primers* / genetics
  • Female
  • Genotype
  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Oligonucleotide Probes* / genetics
  • Papillomaviridae / classification
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification*
  • Papillomavirus Infections / diagnosis*
  • Papillomavirus Infections / virology*
  • Sensitivity and Specificity

Substances

  • DNA Primers
  • Oligonucleotide Probes