Abstract
We describe a novel zebrafish line that fluorescently tags a previously unknown protein, CT74a, allowing us to follow its endogenous expression in real time and at subcellular resolution in live embryos. Our results showed that CT74a-Citrine fusion protein is expressed in the developing pharyngeal arches, hindbrain, and fin buds in a pattern highly reminiscent of transcription factors belonging to anterior Hox gene families, including expression in a subset of neuronal nuclei. Consistent with this, splinkerette-PCR revealed that CT74a-Citrine's genomic integration is within the HoxB region, and 3' RACE demonstrated that its downstream coding sequence has no recognizable homology. Thus, CT74a is a previously unknown protein located within the HoxB cluster adjacent to Hoxb4a and is expressed in a Hoxb4a-like pattern.
Keywords:
HoxB cluster; Hoxb4a; fluorescent fusion protein; zebrafish.
© 2014 Wiley Periodicals, Inc.
Publication types
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Letter
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Genetically Modified / embryology
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Animals, Genetically Modified / genetics
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Animals, Genetically Modified / metabolism
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Bacterial Proteins / metabolism
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Branchial Region / embryology
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Branchial Region / metabolism*
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Cell Nucleus / genetics
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Cell Nucleus / metabolism
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Cytoplasm / genetics
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Cytoplasm / metabolism
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Embryo, Nonmammalian / metabolism
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Gene Expression Regulation, Developmental
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism*
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Luminescent Proteins / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Rhombencephalon / embryology
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Rhombencephalon / metabolism*
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Zebrafish / embryology*
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Zebrafish / genetics
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Zebrafish / metabolism
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Zebrafish Proteins / genetics
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Zebrafish Proteins / metabolism*
Substances
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Bacterial Proteins
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Homeodomain Proteins
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Luminescent Proteins
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Recombinant Fusion Proteins
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Zebrafish Proteins
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citrine protein, bacteria
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unm_ct74a protein, zebrafish