Smart magnetic nanoparticle-aptamer probe for targeted imaging and treatment of hepatocellular carcinoma

Chalermchai Pilapong; Sudarat Sitthichai; Somchai Thongtem; Titipun Thongtem

doi:10.1016/j.ijpharm.2014.07.036

Smart magnetic nanoparticle-aptamer probe for targeted imaging and treatment of hepatocellular carcinoma

Int J Pharm. 2014 Oct 1;473(1-2):469-74. doi: 10.1016/j.ijpharm.2014.07.036. Epub 2014 Jul 30.

Authors

Chalermchai Pilapong¹, Sudarat Sitthichai², Somchai Thongtem², Titipun Thongtem³

Affiliations

¹ Center of Excellence for Molecular Imaging (CEMI), Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: chalermchai.pilapong@cmu.ac.th.
² Department of Physics and Material Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
³ Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.

PMID: 25089503
DOI: 10.1016/j.ijpharm.2014.07.036

Abstract

We report herein the development of a smart magnetic nanoparticle-aptamer probe, or theranostic nanoprobe, which can be used for targeted imaging and as a drug carrier for hepatocellular carcinoma treatment. The theranostic nanoprobe combines the delivery potential of a non-toxic cellulose derivative polymer, specific capability of cancer-specific molecule (DNA-based EpCAM aptamer) and the imaging capability of magnetic iron oxide nanoparticles. Our proof-of-concept design demonstrates efficient in vitro MR imaging of the cancer cells, and enhanced delivery of an anticancer drug into the cancer cells with comparable treatment efficacy.

Keywords: Drug delivery; EpCAM aptamer; MR imaging; Magnetic nanoparticles; Theranostic nanoplatform.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigens, Neoplasm / genetics*
Antimetabolites, Antineoplastic / administration & dosage*
Antimetabolites, Antineoplastic / chemistry
Aptamers, Nucleotide / administration & dosage*
Aptamers, Nucleotide / chemistry
Carboxymethylcellulose Sodium / administration & dosage*
Carboxymethylcellulose Sodium / chemistry
Carcinoma, Hepatocellular / drug therapy
Carcinoma, Hepatocellular / metabolism
Cell Adhesion Molecules / genetics*
Cell Survival / drug effects
Doxorubicin / administration & dosage*
Doxorubicin / chemistry
Epithelial Cell Adhesion Molecule
Ferric Compounds / administration & dosage*
Ferric Compounds / chemistry
Hep G2 Cells
Humans
Liver Neoplasms / drug therapy
Liver Neoplasms / metabolism
Magnetic Phenomena
Magnetic Resonance Imaging
Metal Nanoparticles / administration & dosage*
Metal Nanoparticles / chemistry

Substances

Antigens, Neoplasm
Antimetabolites, Antineoplastic
Aptamers, Nucleotide
Cell Adhesion Molecules
Epithelial Cell Adhesion Molecule
Ferric Compounds
ferric oxide
Doxorubicin
Carboxymethylcellulose Sodium