Rapid flow through immunoassay for CRP determination based on polyethylene filters modified with ω-aminocellulose carbamate

Macromol Biosci. 2014 Nov;14(11):1539-46. doi: 10.1002/mabi.201400179. Epub 2014 Aug 4.

Abstract

A novel method for antibody immobilization is discussed and applied in a column based flow through immunoassay system (ABICAP). It is shown that porous polyethylene material can be modified with different ω-aminocellulose carbamates yielding an amino group containing biocompatible support for antibody immobilization. Anti-h CRP antibodies can be bound covalently to the surface via various homobifunctional cross-linkers. The antibody modified filters are validated for the CRP determination in a sandwich ABICAP system. In a 10 min test procedure based on colloidal dye particles, a limit of detection of 5 ng CRP mL(-1) and coefficients of variation of <9.1% are obtained.

Keywords: ABICAP; C-reactive protein; biosensors; film formation; ω-aminocellulose carbamate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / metabolism
  • C-Reactive Protein / metabolism*
  • Calibration
  • Carbamates / chemistry*
  • Cellulose / chemistry*
  • Humans
  • Immobilized Proteins / metabolism
  • Immunoassay / methods*
  • Polyethylene / chemistry*

Substances

  • Antibodies
  • Carbamates
  • Immobilized Proteins
  • Polyethylene
  • Cellulose
  • C-Reactive Protein