Single-cell imaging of mechanotransduction in endothelial cells

Prog Mol Biol Transl Sci. 2014:126:25-51. doi: 10.1016/B978-0-12-394624-9.00002-6.

Abstract

Endothelial cells (ECs) are constantly exposed to chemical and mechanical microenvironment in vivo. In mechanotransduction, cells can sense and translate the extracellular mechanical cues into intracellular biochemical signals, to regulate cellular processes. This regulation is crucial for many physiological functions, such as cell adhesion, migration, proliferation, and survival, as well as the progression of disease such as atherosclerosis. Here, we overview the current molecular understanding of mechanotransduction in ECs associated with atherosclerosis, especially those in response to physiological shear stress. The enabling technology of live-cell imaging has allowed the study of spatiotemporal molecular events and unprecedented understanding of intracellular signaling responses in mechanotransduction. Hence, we also introduce recent studies on mechanotransduction using single-cell imaging technologies.

Keywords: Endothelial cells; Mechanotransduction; Single cell imaging.

Publication types

  • Review

MeSH terms

  • Animals
  • Endothelial Cells / metabolism*
  • Focal Adhesions / metabolism
  • Humans
  • Mechanotransduction, Cellular*
  • Molecular Imaging / methods*
  • Single-Cell Analysis / methods*
  • Wound Healing