cDNA cloning of Gb, the substrate for botulinum ADP-ribosyltransferase from bovine adrenal gland and its identification as a rho gene product

Biochem Biophys Res Commun. 1989 Sep 15;163(2):1175-81. doi: 10.1016/0006-291x(89)92344-9.

Abstract

A 1.5 kilobase cDNA coding for the complete amino acid sequence of Gb, the substrate for ADP-ribosyltransferase in C1 and D botulinum toxins from bovine adrenal gland, has been isolated from a cDNA library of bovine adrenal gland. This cDNA encodes a polypeptide of 21,770 Da consisting of 193 amino acid residues, and the deduced amino acid sequence contains all the partial amino acid sequences reported previously (Narumiya, S., Sekine, A., and Fujiwara, M. (1988) J. Biol. Chem., 263, 17255-17257). Sequence comparison revealed that Gb is identical with the product of human rho clone 12 (rho A). The present results also confirmed our suggestion that the ADP-ribosylation occurs at Asn41 in the putative effector domain of the rho gene product.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Glands / enzymology*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Botulinum Toxins
  • Cattle
  • Cloning, Molecular
  • DNA / genetics*
  • GTP-Binding Proteins / metabolism
  • Humans
  • Molecular Sequence Data
  • Poly(ADP-ribose) Polymerases / metabolism*
  • Rho Factor / genetics*
  • Substrate Specificity
  • Transcription Factors / genetics*

Substances

  • Rho Factor
  • Transcription Factors
  • DNA
  • Poly(ADP-ribose) Polymerases
  • Botulinum Toxins
  • GTP-Binding Proteins

Associated data

  • GENBANK/M27278